Biosynthesis of Lactose by Mammary Gland Slices from the Lactating Rat
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چکیده
منابع مشابه
Biosynthesis of lactose by mammary gland slices from the lactating rat.
The incorporation of the isotope from D-glucose-lJ4C, D-glucose-l-3H, D-glucose-Z J4C, D-ghcose-3-3H, D-glucose3 ,4-14C, D-glucose-6-14C, D-glucose-6-3H, uniformly labeled D-glucoseJ4C, uniformly labeled D-galactoseJ4C, and D-mannose-lJ4C into lactose by slices of mammary glands of lactating rats (14 to 20 days post partum) was studied. The lactose formed was hydrolyzed either with acid or with...
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Because vitamin A in milk is largely present as esterified retinol while blood plasma predominantly contains unesterified retinol, experiments were conducted to determine whether membranes from the lactating mammary gland are able to synthesize retinyl esters in vitro. When microsomes from rats lactating for 7 to 14 days were incubated with [(3)H]retinol dispersed in dimethylsulfoxide, some [(3...
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Lipogenesis from propionate-l-Cl4 was first reported by Feller and Feist (2) in slices of liver and adipose tissue. Favarger and Gerlach (3, 4) and Masoro and Porter (5) recently studied the incorporation of propionate-1-V into fatty acids of rat and mouse adipose tissue in viva and in vitro. The incorporation of propionate, compared with that of acetate, was quite low. Favarger and Gerlach (4)...
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Mammary gland utilizes a combination of substrates in vivo. To determine if and how the substrates presented to the gland alter milk fat synthesis, slices of mammary gland of lactating rats were incubated with combinations of glucose, L-lactate, and pyruvate. Uptakes and conversions of uniformly and specifically labeled substrates to CO2 and fatty acid were measured. The absolute rate of fatty ...
متن کاملThe lactose synthetase particles of lactating bovine mammary gland. Preparation of particles with intact lactose synthetase.
1. The particulate form of lactating bovine mammary lactose synthetase activity is shown to be more highly organized than previously reported. 2. A novel method of shattering frozen mammary tissue with effective cell disruption is described. 3. The apparent subcellular distribution of lactose synthetase was shown to reflect the method of homogenization. 4. After mild homogenization particles as...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96812-2